Beekeeping Situation and Perspective in Córdoba Province Tasinato Method of Queen Cells Production |
Antibacterial activity of Monospecific Honeys of Algarrobo (Prosopis spp.), Eucalyptus and Chilca (Baccharis spp.) against Meticiline Resistant Bacteria of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa Fernando L. Esteban Córdoba - Argentina PRESENTATION "This work, carries out a very significant contribution to the studies of the honey antibacterial power and very original, because it correlates the honey composition and characteristic with its inhibitory activity against bacteria. As you can see, it was found very interesting data, and correlation that could serve as a base for studies to come". José Francisco Maidana Director of CEDIA (Beekeeping Investigation Center - Santiago del Estero - Argentina) February 29th 2008 ABSTRACT In terms of its strong organoleptic characters and high concentration of monospecific pollen there were selected three different samples of honeys crop 2006-2007: Eucalyptus sp.; Baccharis sp. and Prosopis sp. of certain areas of the Argentina. The antibacterial activity of this honeys was determined by the World Health Organization techniques in front of meticiline resistant strains of Staphylococcus aureus ATCC 29213; Pseudomonas aeruginosa ATCC 227853 and Escherichia coli ATCC 35218. For each experiment it was prepared honey dilutions of 100%, 75%, 50%, 25% and 12,5% volume in volume. It was prepared a sugar dilution with the same concentration of honey to compare each experiment. The antibacterial activity of each honey was related with the Physicochemical parameters of Humidity and Soluble Solids, HMF, electric Conductivity, free Acidity, pH, diastase Index, glucoxidase Activity, catalase Detection, peroxidase Detection and Colour. Honey samples of Eucalyptus (Eucalyptus sp.), Chilca (Baccharis sp.) and Algarrobo (Prosopis sp.) submited to study, presented antibacterial activity in front of meticiline resistant strains of Staphylococcus aureus ATCC 29213, Pseudomonas aeruginosa ATCC 227853 and Escherichia coli ATCC 35218. The osmotic pressure was discarded as the only honey antibacterial factor. Different results of each honey samples and their dissolutions in front of each bacterial strain was proven. The antibacterial activity of this honeys and their physicochemical characters was compared. Gucoxidase activity was detected in the three samples by hydrogen peroxide presence, considered main antibacterial factor of honeys. In those samples where the catalase activity was positive, its presence would be proportional to the diastase activity and restrictive of the glucoxidase activity. The antibacterial activity didn't keep a directly proportional relationship with the hydrogen peroxide production. In order of the obtained results it could be recommend the sugar substitution for these honeys, to prepare wound dressings, as well as their possible use as food preserve additive. Fernando Luis Esteban To top |
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